Glucoraphanin, the precursor of the widely extolled chemopreventive agent sulforaphane found in broccoli, has antioxidant activity.

Glucoraphanin 在高脂肪饮食(HFD)喂养的小鼠中减少体重增加并增加能量消耗。它能提高HFD喂养的小鼠的胰岛素敏感性和葡萄糖耐受性。然而，在Nrf2/小鼠中，Glucoraphanin不展现出抗肥胖和增强胰岛素敏感性的效果。Glucoraphanin能阻断HFD诱导的野生型小鼠白色脂肪组织中Ucp1蛋白水平的减少，但在Nrf2/小鼠中无此效果。Glucoraphanin缓解HFD诱导的肝脏脂肪变性和氧化应激。它抑制HFD诱发的肝脏和脂肪组织中巨噬细胞的促炎症激活[1]。包含0.1% Glucoraphanin (GF)的颗粒显著减轻了压力小鼠的社交回避时间降低。在1%蔗糖偏好测试(SPT)中，包含0.1% GF的处理显著减轻了压力小鼠的蔗糖偏好下降[2]。

Male C57BL/6JSlc mice are 7 weeks of age. The Nrf2 knockout (Nrf2?/?) mouse strain is used in the assay. After 1 week of acclimation, mice are fed normal chow (NC) (containing 2.2% dextrinized cornstarch, 10% kcal from fat), NC containing 0.3% glucoraphanin (NC-GR) (containing 2.2% extract powder), an HFD (containing 2.2% dextrinized cornstarch, 60% kcal from fat), or an HFD containing 0.3% glucoraphanin (HFD-GR) (containing 2.2% extract powder) for 14 weeks. Both the NC and the HFD containing cornstarch or glucoraphanin are prepared. All mice studied are maintained on a 12-h light/dark cycle at 24-26°C with free access to water and food [1].