Nucleozin targets influenza A nucleoprotein (NP), a multifunctional, RNA-binding protein necessary for virus replication.

Nucleozin 抑制了已经进行过核输出的核糖核蛋白(RNPs)在胞质的运输，促进了这些RNPs与细胞内的Rab11形成大型围核聚集体。该作用导致了病毒颗粒数量显著减少，而且往往体积更小。Nucleozin 的主要靶点是病毒的 RNP。通过阻断病母体基因组在胞质的运输，可以有效地抑制 IAV 的复制[1]。

Nucleozin通过诱导NP聚集和抑制其在核内积累，阻碍了流感A病毒在体外的复制，并且以nM级的中位有效浓度(EC(50))显示出效果，能够保护小鼠免受致死剂量的禽流感A H5N1的挑战。研究表明，病毒的NP是开发小分子疗法的有效靶点[2]。

For live imaging, cells were grown in chambered glass bottom dishes and maintained at 37°C in L-15 CO2-independent medium (Gibco) during analysis. Cells were transfected with GFP-NP and infected with PR8 12 h later. For nucleozin treatment, images were acquired for 5 min and then nucleozin was added to a final concentration of 2 μM before imaging for around 20 to 30 min . Images were acquired at 0.25 or 0.71 frame/s and processed with ImageJ[1].