Corticosterone (Kendall's compound B) is an adrenocortical steroid with salocorticoid and glucocorticoid activity that is orally active. Corticosterone is involved in the regulation of energy, immune responses, and stress responses in the body.

方法：小鼠乳腺增生性上皮细胞株 TM10 用 Corticosterone (50-200 µM) 处理 24-72 h，使用 crystal violet staining 检测细胞数。
结果：Corticosterone 显著抑制细胞生长，50、100、200 µM 处理 72 h 后，细胞生长被抑制 50、57、76%。[1]
方法：人神经母细胞瘤细胞 SK-N-BE(2)C 用 Corticosterone (1-100 nM) 处理 1-14 天，使用 RT-PCR 检测基因表达水平。
结果：Corticosterone 增加 SK-N-BE(2)C 细胞中 NET mRNA 水平。[2]

方法：为研究对小鼠的抑郁样行为的影响，将 Corticosterone (20 mg/kg，suspended in physiological saline containing 0.1% DMSO and 0.1% Tween-80) 皮下注射给 C57BL/6N 小鼠，每天一次，持续 1-5 周。
结果：强迫游泳和尾部悬吊试验中，重复注射 Corticosterone 以时间依赖的方式增加了不动行为。同时，这种注射模式对小鼠海马中的酪氨酸羟化酶水平产生了时间相关的影响。这些结果与应激诱导的抑郁症模型中的相关性一致。[3]
方法：为研究对恐惧的调节作用，将 Corticosterone (2 mg/kg，2.5% EtOH in saline) 腹腔注射给进行听觉恐惧条件反射范式训练的 C57Bl/6 小鼠。
结果：Corticosterone 影响记忆巩固和恢复，在雄性小鼠中，Corticosterone 持续增加对音调的冷冻行为，而在雌性小鼠中，训练后 24 小时 Corticosterone 减少了冷冻行为。[4]

HEK293 cells are grown in 6-cm dishes in 10% fetal bovine serum DMEM medium. When cells are 90% confluent, the medium is changed to 0.5% fetal bovine serum DMEM to limit serum-induced up-regulation of SGK. For in vitro phosphorylation analysis, the following approach is used. HEK293 cells are transfected with or without SGK1 small interfering RNA. One day after transfection, cells are treated without or with 100 nM corticosterone for 30 min, washed, and maintained in 0.5% fetal bovine serum DMEM for 1.5 h. Then cells are lysed in the CytoBuster protein extraction reagent containing protease inhibitors. Cell lysates are centrifuged at 16,000 × g at 4 °C for 20 min. The supernatants (40 μl, ～50 μg of total protein) are incubated with 1 μg of purified GST fusion protein of wild-type GDI or its mutants for 30 min at 30 °C in the reaction buffer (30 mM HEPES, pH 7.5, 10 mM MgCl2, 30 μM ATP, 1 μCi of [γ-32P]ATP, 100 nM calyculin, 1 μM okadaic acid). SDS-PAGE is carried out, and phosphorylated GDI is visualized with autoradiography.(Only for Reference)