Buparvaquone (BW 720C) is a hydroxynaphthoquinone antiprotozoal drug related to atovaquone and parvaquone.

Buparvaquone对L. (L.) infantum chagasi细胞内阿米巴原虫具有显著的选择性，其IC50值为1.5 μM。在为期4天的增殖实验中，buparvaquone有效抑制N.caninum裂殖子体的复制（IC50=4.9nM；IC100=100nM）。其他皮肤种类也对buparvaquone敏感，其IC50值范围在1-4 μM之间。

水合凝胶和含有buparvaquone的水包油乳剂均可明显降低皮肤寄生虫负担和病损大小，效果优于未经处理的对照组。使用buparvaquone（100 mg/kg）治疗N. caninum感染的小鼠，无论是通过腹腔注射还是灌胃给药，均可有效预防新孢子虫病的症状，在腹腔注射组的6只小鼠中有4只得到防治，在口服治疗组的7只小鼠中有6只得到防治。

To study whether pretreatment of host cells prior to invasion had any effect on parasite proliferation, confluent HFF grown in 6-well plates are treated with 1?μM buparvaquone in medium for 1?h or 5?h, and controls are exposed to the corresponding amounts of DMSO. Subsequently, the drug-containing medium is removed and monolayers are ished 4 times with Hank's Balanced Salt Solution, and are infected with Nc-Liv tachyzoites in 5?mL medium without any drug or solvent. After 2 days, cells are collected with a cell scraper, centrifuged, ished once more in PBS, and the pellet is stored at ?20?°C prior to quantification of N.?caninum proliferation by N.?caninum-specific real time PCR as outlined below.

Mice: On day 0, all mice are infected by intraperitoneal (i.p.) injection of freshly purified N.?caninum tachyzoites. After 48?h, mice receive BPQ (100?mg/kg) as suspension in corn oil either by i.p. injection of a volume of 100?μl or by oral application of 100?μl by gavage. The control groups obtained the corresponding amount of the solvent only, either i.p. or orally. The treatments are performed 5 times on a daily basis. If not indicated otherwise, mice are inspected twice daily for clinical signs (ruffled coat, apathy, hind limb paralysis) until day 21 post infection (p.i.), at which time they are euthanized.