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Aprotinin (Traskolan) 是一种广谱丝氨酸蛋白酶 (BPTI) 抑制剂,可抑制多种不同酯酶和蛋白酶的活性。Aprotinin 是一种抗纤溶剂,用于减少复杂手术中的出血。
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Aprotinin (Traskolan) 是一种广谱丝氨酸蛋白酶 (BPTI) 抑制剂,可抑制多种不同酯酶和蛋白酶的活性。Aprotinin 是一种抗纤溶剂,用于减少复杂手术中的出血。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
5 mg | ¥ 410 | 现货 | |
10 mg | ¥ 492 | 现货 | |
25 mg | ¥ 1,060 | 现货 | |
50 mg | ¥ 1,880 | 现货 | |
100 mg | ¥ 2,890 | 现货 | |
200 mg | ¥ 3,590 | 现货 | |
500 mg | ¥ 5,790 | 现货 |
产品描述 | Aprotinin (Traskolan) is a broad-spectrum serine protease (BPTI) inhibitor that inhibits the activity of a number of different esterases and proteases. Aprotinin is an antifibrinolytic agent used to minimize hemorrhage during complex surgical procedures. |
靶点活性 | Trypsin:0.06 pM(Kd), Chymotrypsin:9.5 nM(Kd), Kallikrein:0.8 nM(Kd), Trypsinogen:2 μM(Kd) |
体外活性 | 方法:SARS-CoV-2 感染的 Caco2 细胞用 Aprotinin (0-20 µM) 处理 48 h,检测细胞病变效应 (CPE)。 结果:Aprotinin 显示出抗病毒作用,对 SARS-CoV-2 感染的 Caco2 细胞 CPE 形成的 IC50 范围为 0.81 µM-1.03 µM。[1] 方法:VSMCs 细胞用 Aprotinin (1-100 µM) 预处理 1 h,用细胞因子 (10 ng/mL IL-1β+25 ng/ml TNF-α) 处理 12 h,通过 Western Blot 和 RT-PCR 检测靶点基因和蛋白表达水平。 结果:Aprotinin 以浓度依赖的方式与细胞因子刺激 HO-1 蛋白质水平和 mRNA 水平的显著增加。[2] |
体内活性 | 方法:为研究体内抗流感病毒活性,将 Aprotinin (2 mg/kg) 静脉注射给致死性 A/PR/8/34 (H1N1) 病毒感染的 C57BL/6 小鼠,每天两次,持续 5 天。 结果:单独使用 Aprotinin 不会导致小鼠体重减轻。PBS 处理的小鼠在感染后 8 天存活率为 0%。同时,用 Aprotinin 治疗的小鼠组显示 75% 的存活率。[3] |
激酶实验 | Substrates and kinases are diluted in 50?mM Tris/HCl (pH?7.5), 0.1% 2-mercaptoethanol, 0.1?mM EGTA and 10?mM magnesium acetate. Reactions are initiated with [γ-32P]ATP (2500 c.p.m./pmol) to a final concentration of 0.1?mM and terminated after 15?min at 30°C by the addition of SDS and EDTA (pH?7.0) to final concentrations of 1.0% (w/v) and 20?mM respectively. After heating for 5?min at 100°C and separation by SDS/PAGE, the phosphorylated proteins are detected by autoradiography. |
细胞实验 | Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days. (Only for Reference) |
别名 | 抑酶肽, 抑肽酶, Bovine Pancreatic Trypsin Inhibitor, Traskolan, Antilysin |
分子量 | 6511.51 |
分子式 | C284H432N84O79S7 |
CAS No. | 9087-70-1 |
存储 | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||
溶解度信息 | H2O: 100 mg/mL (15.36 mM), Sonication is recommended. | ||||||||||||||||||||
溶液配制表 | |||||||||||||||||||||
H2O
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