hCAIX-IN-16 (Compound 12d), an inhibitor of hCA IX, exhibits inhibition constants (K i) of 190.0 nM for hCA IX and 187.9 nM for hCA XII. It can arrest the cell cycle in the G0-G1 and S phases and induce apoptosis in MDA-MB-468 breast cancer cells. Demonstrating broad-spectrum anticancer activity, hCAIX-IN-16 is a potential tool for cancer research [1].

hCAIX-IN-16 (3.99 μM；24小时) 在MDA-MB-468细胞中阻断G0-G1及S期的细胞生长[1]。hCAIX-IN-16 (10 µM) 抑制了44种癌细胞系的生长，其中GI% ≥ 10%，并且对于白血病（CCRF-CEM、RPMI-8226和SR）及乳腺癌细胞系（T-47D和MDA-MB-468）展现出GI% ≥ 50%的效果[1]。hCAIX-IN-16 在MDA‑MB‑468细胞中通过凋亡机制引发细胞死亡[1]。在低氧条件下，抑制MDA-MB-468和CCRF-CM细胞，其IC 50分别为3.99和4.51 µM[1]。