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CW-069

产品编号 T6209Cas号 1594094-64-0
别名 CW069

CW-069 是一种微管运动蛋白 HSET 的变构选择性抑制剂,IC50值为 75 μM,对 KSP 具有显着的特异性。

CW-069
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CW-069

纯度: 98.59%
产品编号 T6209 别名 CW069Cas号 1594094-64-0

CW-069 是一种微管运动蛋白 HSET 的变构选择性抑制剂,IC50值为 75 μM,对 KSP 具有显着的特异性。

规格价格库存数量
1 mg¥ 346现货
5 mg¥ 825现货
10 mg¥ 1,260现货
25 mg¥ 2,270现货
50 mg¥ 3,170现货
100 mg¥ 4,450现货
500 mg¥ 9,290现货
1 mL x 10 mM (in DMSO)¥ 889现货
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纯度:98.59%
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产品介绍

生物活性
产品描述
CW-069 (IC50=75 μM), an allosteric selective inhibitor of microtubule motor protein HSET, exhibits remarkable specificity over KSP.
靶点活性
HSET:75 μM
体外活性
CW069在具有多余中心体的N1E-115细胞中增加多极纺锤体,但不改变正常人类真皮成纤维细胞的双极纺锤体形态。同时,CW069对N1E-115癌细胞的生长具有抑制作用,IC50为10 μM。[1]
激酶实验
In Vitro Enzymatic ATPase Assay: The protocol is optimized for use with full-length, N-terminal, 6His-tagged HSET and KSP, and measured the MT-stimulated activity of the proteins. Inhibition of the Gsp synthetase activity of HSET/KSP is observed spectrophotometrically by coupling the hydrolysis of ATP to oxidation of NADH via pyruvate kinase/lactate dehydrogenase reactions. The assay is initiated by adding purified Gsp synthetase/amidase (12.8 nM) to an assay mixture containing the following components (final concentration): 6 nM protein, 0.07 mg/ml MTs (University Biologicals), 1.56 mM glutathione, 10 mM spermidine, 2 mM ATP, 2.7 mM MgCl2, 1 mM phospho(enol)-pyruvate, 0.2 mM NADH, 50 μg/ml lactate dehydrogenase, 100 μg/ml pyruvate kinase, and various concentrations of inhibitor all in 50 mM Na PIPES (pH 6.8) at 37°C. The ADP-Glo detection assay (Promega) is performed as described in the manufacturer's instructions. All compound additions were performed using a multidrop BioMek Nxp. Plates were read using a Pherastar microplate reader.
细胞实验
Cells are cultured in DMEM supplemented with 10% fetal calf serum (FCS) at 37°C and 5% CO2. All compounds used in the Sulforhodamine B colorimetric (SRB) assay are dissolved in DMSO and diluted in culture medium to a final concentration of 0.2% DMSO. For the SRB assay and live-cell imaging, cells are seeded in 96-well plates at a density of 2,500 cells per well. After 24 hr, the cells are treated with compound for 72 hr, with triplicate wells for each concentration. For the SRB assay, the cells are then fixed with trichloroacetic acid (TCA) and stained with SRB. Fluorescence is quantified using an Infinite 200 PRO plate-reader at a wavelength of 545 nm. Compound-treated wells are compared with solvent control wells and the concentration of compound that results in 50% of the solvent-control cell growth is designated as the IC50 concentration, calculated using Graphpad PRISM 6. At least three biological replicates are performed for each assay.(Only for Reference)
别名CW069
化学信息
分子量500.33
分子式C23H21IN2O3
CAS No.1594094-64-0
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
Ethanol: 4 mg/mL (7.99 mM)
DMSO: 93 mg/mL (185.9 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
溶液配制表
Ethanol/DMSO
1mg5mg10mg50mg
1 mM1.9987 mL9.9934 mL19.9868 mL99.9340 mL
5 mM0.3997 mL1.9987 mL3.9974 mL19.9868 mL
DMSO
1mg5mg10mg50mg
10 mM0.1999 mL0.9993 mL1.9987 mL9.9934 mL
20 mM0.0999 mL0.4997 mL0.9993 mL4.9967 mL
50 mM0.0400 mL0.1999 mL0.3997 mL1.9987 mL
100 mM0.0200 mL0.0999 mL0.1999 mL0.9993 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

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关键词

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