Euphol, an alcohol tetracyclic triterpene, has a wide range of pharmacological properties and is considered to have anti-inflammatory action.

经口给予euphol (30 和 100 mg/kg) 能减轻由卡拉胶引起的机械性痛觉过敏。同样，通过脊髓和脑室内途径给药的euphol也能预防卡拉胶引起的机械性痛觉过敏。euphol一致性地阻断了由完全弗氏佐剂、角质形成细胞来源的趋化因子、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α引起的机械性痛觉过敏，并伴随着小鼠爪中髓过氧化物酶活性的抑制。经口给予的euphol在预防坐骨神经结扎引起的机械性伤害反应方面也有效，并且在完全弗氏佐剂i.pl.注射后显著降低了爪和脊髓组织中的细胞因子/趋化因子水平及mRNA。此外，用CBR或CBR拮抗剂预处理，显著逆转了euphol的镇痛效果。

For the induction of inflammatory pain, mice received an intraplantar injection of 20 ml of carrageenan (300 ug/paw) under the surface of the right hindpaw.?To assess the systemic effect of drug treatment, mice received euphol (3, 30 or 100 ug/kg) or its vehicle (5% Tween 80 solution made in saline e 0.9% NaCl solution), 1 h before carrageenan injection.?Other group of mice received dexamethasone (0.5 mg/kg.) 1 h before carrageenan?injection that was used as positive control drug.?The development of mechanical hyperalgesia was evaluated at 1, 2, 4, 6, 8, 24 and 48 h time points, after drug pre-treatments.?In order to evaluate the possible site of action of euphol (central or peripheral), a separate group of animals received an i.pl.?injection of euphol (5, 10 or 30 ug/paw) 15 min prior carrageenan (300 ug/paw) administration.?Additional groups of animals received an intrathecal (1, 3 and 10 ug/site) or intracerebroventricular ?(0.3, 3 or 10 ug/site) injection of 5 ml of euphol, 15 min before the paw injection of carrageenan.?Compared the analgesic efficacy of euphol with different groups of standard analgesic drugs, as described previously: indomethacin (2 mg/kg), morphine (5 mg/kg.), gabapentin (70 mg/kg) and dipyrone (120 mg/kg).?All drugs were administered 1 h before i.pl.?carrageenan injection (300 ug/paw).?Mechanical hyperalgesia was evaluated at 1, 2, 4, 6, 8 and 24 h time points after carrageenan injection, and measured with VFH.