购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

AZD-2461

产品编号 T2484Cas号 1174043-16-3

AZD-2461 是一种 PARP 抑制剂,可抑制 PARP1、PARP2 和 PARP3 的活性,IC50值分别为 5、2 和 200 nM。

AZD-2461
TargetMol

为众多的药物研发团队赋能,

让新药发现更简单!

AZD-2461

纯度: 99.25%
产品编号 T2484Cas号 1174043-16-3

AZD-2461 是一种 PARP 抑制剂,可抑制 PARP1、PARP2 和 PARP3 的活性,IC50值分别为 5、2 和 200 nM。

规格价格库存数量
5 mg¥ 457现货
10 mg¥ 671现货
50 mg¥ 1,232现货
100 mg¥ 1,996现货
1 mL x 10 mM (in DMSO)¥ 498现货
大包装 & 定制
加入购物车
实验操作小课堂
查看更多

"AZD-2461"的相关化合物库

选择批次:
纯度:99.25%
联系我们获取更多批次信息
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。

产品介绍

生物活性
产品描述
AZD2461 is a novel PARP inhibitor.
靶点活性
PARP3:200 nM, PARP2:2 nM, PARP1:5 nM
体外活性
AZD2461在抗Olaparib的KB1P肿瘤T6-28上Mdr1b表达增加了80倍,没有抑制Pgp.
体内活性
AZD2461比奥拉帕尼对Pgp的亲和力低。
激酶实验
ELISA-Based ERBB Kinase Assay: The ERBB1, ERBB 2, and ERBB4 cytoplasmic fusion proteins are made by cloning the ERBB1 sequence (Met-668 to Ala-1211), ERBB2 (Ile-675 to Val-1256), and ERBB4 sequence (Gly-259 to Gly-690) into the baculoviral vector pFastBac using PCR. Proteins are expressed in baculovirusinfected Sf9 insect cells as GST fusion proteins. The proteins are purified by affinity chromatography using glutathione sepharose beads. Inhibition of ERBB tyrosine kinase activity is assessed using an ELISA-based receptor tyrosine kinase assay. Kinase reactions (50 mM HEPES, pH 7.4, 125 mM NaCl, 10 mM MgCl2, 100 μM sodium orthovanadate, 2 mM dithiothreitol, 20 μM ATP, PF299804 or vehicle control, and 1-5 nM GST-erbB per 50 μL of reaction mixture) are run in 96-well plates coated with 0.25 mg/mL poly-Glu-Tyr. The reactions are incubated for 6 minutes at room temperature while being shaken. Kinase reactions are stopped by removal of the reaction mixture, and then the wells are washed with wash buffer (0.1% Tween 20 in PBS). Phosphorylated tyrosine residues are detected by adding 0.2 μg/mL antiphosphotyrosine antibody (Oncogene Ab-4; 50 μL/well) coupled to horseradish peroxidase (HRP) diluted in PBS containing 3% BSA and 0.05% Tween 20 for 25 minutes while being shaken at room temperature. The antibody is removed, and plates are washed in wash buffer. HRP substrate (SureBlue3,3?,5,5?-tetramethyl benzidine or TMB) is added (50 μL per well) and incubated for 10-20 minutes while it is shaken at room temperature. The TMB reaction is stopped with the addition of 50 μL of stop solution (0.09 N H2SO4). The signal is quantified by measuring absorbance at 450 nm. IC50 values are determined for PF299804 using the median effect method.
化学信息
分子量395.43
分子式C22H22FN3O3
CAS No.1174043-16-3
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
DMSO: 45 mg/mL (113.8 mM)
Ethanol: 39.5 mg/mL (100 mM)
溶液配制表
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.5289 mL12.6445 mL25.2889 mL126.4446 mL
5 mM0.5058 mL2.5289 mL5.0578 mL25.2889 mL
10 mM0.2529 mL1.2644 mL2.5289 mL12.6445 mL
20 mM0.1264 mL0.6322 mL1.2644 mL6.3222 mL
50 mM0.0506 mL0.2529 mL0.5058 mL2.5289 mL
100 mM0.0253 mL0.1264 mL0.2529 mL1.2644 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

对于不同动物的给药剂量换算,您也可以参考 更多

关键词

Related Tags: buy AZD-2461 | purchase AZD-2461 | AZD-2461 cost | order AZD-2461 | AZD-2461 chemical structure | AZD-2461 in vivo | AZD-2461 in vitro | AZD-2461 formula | AZD-2461 molecular weight