N6-Methyladenosine is a methylated adenine residue, glycoside is an endogenous uridine product of transfer RNA degradation. It is the most prevalent internal modification of messenger RNA present in all higher eukaryotes and can modify viral RNA with antiviral activity.

N6-Methyladenosine是一种在mRNA中广泛存在的修饰，也发现于一些病毒及大多数真核生物中。同样在tRNA、rRNA和小核RNA（snRNA）以及一些长非编码RNA中发现，比如Xist。[1] [2] [3] N6-Methyladenosine是转移核糖核酸（tRNA）降解的内源性尿核苷产物。[4] 在许多组织中，N6-Methyladenosine是一种广泛的RNA修饰，尤其在大脑中含量高。N6-Methyladenosine在小鼠和人类mRNAs的终止密码子附近以及3'UTRs中有所富集。[5] 最近的研究发现，肥胖风险基因FTO编码的是一种m6A去甲基化酶，暗示m6A是生理过程中的一个重要调节器。[6]

LD50：小鼠>1 g/kg（经口给药）。[7]

NOD1 Dose Response assay: Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with Water. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.