L-Leucyl-L-Leucine methyl ester hydrochloride (Leu-Leu-ome hydrochloride) is a dipeptide condensation product of L-leucine methyl ester produced by human monocytes or polymorphonuclear leukocytes. L-Leucyl-L-Leucine methyl ester hydrochloride selectively eliminates lymphocytes with cytotoxic potential and also induces lysosomal stress.

方法：HeLa 细胞用 L-Leucyl-L-Leucine methyl ester hydrochloride (1-7 mM) 处理 18 h，通过 Crystal Violet staining 检测细胞数。
结果：L-Leucyl-L-Leucine methyl ester hydrochloride 诱导细胞死亡，但仅在 4 mM 以上的浓度下。[1]
方法：T. brucei 细胞用 L-Leucyl-L-Leucine methyl ester hydrochloride (30 µM) 处理 1-4 h，通过 immunofluorescence assay 监测溶酶体形态。
结果：经 L-Leucyl-L-Leucine methyl ester hydrochloride 处理后，p67 的溶酶体染色变得更加分散和破碎，如连续光学切片的二维投影所示。对图像进行定量，发现含有破碎溶酶体的细胞百分比从 t=0 时的 12% 增加到 2 h 和 3 h 时的 >40%。[2]