Hypocrellin A is a natural PKC inhibitor with light-induced antitumor, antifungal and antiviral activities and also exerts immunomodulatory effects on MHC-restricted antigen presentation.

方法：用0.01μM，0.1μM，0.5μM，1μM，5μM，10μM，50μM，100μMHA处理三种 NSCLC 细胞系 H460、PC-9和H197548小时，测定IC50。
结果：Hypocrellin A对H1975， H460，PC-9的IC50分别是0.2223μM、0.2706μM和0.6223μM。[1]
方法：在0.08 μmol/L Hypocrellin A-PDT 24 h处理后12或24小时收集A549细胞，然后测定细胞凋亡，通过SDS-PAGE分离细胞裂解物的等分试样，并通过蛋白质印迹法分析裂解的PARP-1水平。
结果：Hypocrellin A 介导的 PDT 显著增加了 A549 细胞的凋亡群体，集体表现为增加了S期和G2-M期的细胞比例，同时降低了G0-G1期的细胞比例，且PARP 裂解在 Hypocrellin A PDT 后 3 h 开始发生。[2]
方法：用Hypocrellin A浓度分别为0.01、0.1、1.0和10µg/ml处理肥胖Zucker大鼠胰岛素靶组织中肝脏和比目鱼骨骼肌部分的离体组织，对PKC酶的活性进行了测定。
结果：Hypocrellin A对肝脏PKC活性的IC50值为0.07µg/ml，对比目鱼肌PKC活性的IC50值为0.26µg/ml。[3]

方法：H460细胞移植到无胸腺雌性BALB/c裸鼠中，每隔一天对实验组的小鼠腹腔注射2.5 mg/kg和5 mg/kg Hypocrellin A，观察小鼠肿瘤体积的大小。
结果：Hypocrellin A以浓度依赖性方式降低了NSCLC肿瘤的重量和体积。[1]

Cells were seeded in 96-well plates at a density of 1*10^4 cells in a final volume of 100 μl and maintained in DMEM medium at 37℃ ?.?After cells were treated with different concentrations of HA (5, 10, 20, 40, 80 μM), 20 μl 5 mg/ml MTT solution were added to each well and incubated for 4 h in the incubator.?Then 150 μl DMSO were added to each well to dissolve the pellet, keep it in the dark for 15 min, and dissolve the crystal completely.?Automatic microplate reader measured at 490 nm optical density (OD value).?The experiment was repeated three times.?For combination test, cells were treated with 20 μM Hypocrellin A?and different?concentrations of OA(25,50,100, 200 μM) .