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GSK2334470是一种高效的、特异性的PDK1抑制剂,IC50=10 nM。
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GSK2334470是一种高效的、特异性的PDK1抑制剂,IC50=10 nM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 198 | 现货 | |
5 mg | ¥ 461 | 现货 | |
10 mg | ¥ 742 | 现货 | |
25 mg | ¥ 1,380 | 现货 | |
50 mg | ¥ 2,480 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 817 | 现货 |
产品描述 | GSK2334470 is a novel PDK1 inhibitor (IC50: ~10 nM, in a cell-free assay), with no inhibitory at other close related AGC-kinases. |
靶点活性 | PDK1:10 nM |
体内活性 | PDK1抑制剂(PDKi)GSK2334470的效果在新生的BrafV600E::Pten/小鼠中通过系统性给予4-HT进行测试。每周两次给予PDK1,显著抑制色素性病变和随之而来的黑色素瘤的发生,同时显著抑制肺转移(通过H&E染色定量分析约80%)和淋巴结转移(通过S100免疫染色),与Pdk1基因敲除所见表型相似。 |
激酶实验 | FGFR1-4 Biochemical Assays: FGFR kinase inhibition assays are performed at KM for ATP. Picomolar to low nanomolar concentrations of FGFR proteins are incubated in 1× Kinase Reaction Buffer (KRB) with 1 μM of CSKtide and 50 to 250 of μM ATP at 25°C for 90 minutes in the presence or absence of a dosed concentration series of inhibitor. All reactions are terminated by the addition of Stop buffer, and plates are read on a Caliper EZReader2. IC50 values are fit with a four-parameter log[Inhibitor] versus response model with floating Hill Slope. |
细胞实验 | GSK2334470 is dissolved in DMSO and diluted with appropriate medium before use. To study the inhibitory effect of GSK2334470 on mTOR-S6K pathway, non-resistant cells and the resistant sublines are treated with GSK2334470 at 5 μM for 1.5 and 12 h in 10 % FBS medium with/without MK-2206 (5 μM)[2]. |
分子量 | 462.59 |
分子式 | C25H34N8O |
CAS No. | 1227911-45-6 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
溶解度信息 | Ethanol: 83 mg/mL (179.4 mM) DMSO: 83 mg/mL (179.4 mM) | |||||||||||||||||||||||||||||||||||
溶液配制表 | ||||||||||||||||||||||||||||||||||||
Ethanol/DMSO
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