Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Fusidic acid (Fusidine) 是一种从 Fusidium coccineum 发酵液中分离出来的甾体类抗生素。它通过阻止核糖体释放翻译延长因子 G 来抑制细菌的生长。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 mg | ¥ 140 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 140 | 现货 |
产品描述 | Fusidic acid (Fusidine) is an antibiotic isolated from the fermentation broth of Fusidium coccineum. |
激酶实验 | DHODase activity is measured by the DCIP colorimetric assay. This is a coupled assay in which oxidation of DHO and subsequent reduction of ubiquinone are stoichiometrically equivalent to the reduction of DCIP. Reduction of DCIP is accompanied by a loss of absorbance at 610 nm (ε=21500 M/cm). The assay is performed in a 96-well microtiter plate at ambient temperature (ca. 25°C). Stock solutions of 10 mM leflunomide and A771726 are prepared in dimethyl sulfoxide (DMSO) and these are diluted with reaction buffer (100 mM Tris and 0.1 % Triton X-100, pH 8.0) to prepare working stocks of the inhibitors at varying concentrations. For each reaction, the well contained 10 nM DHODase, 68 μM DCIP, 0.16 mg/mL gelatin, the stated concentration of ubiquinone, 10 μL of an inhibitor working stock to give the stated final concentration, and reaction buffer. After a 5-min equilibration period, the reaction is initiated by addition of DHO to the stated final concentrations. The total volume of reaction mixture for each assay is 150 μL, and the final DMSO concentration is ≤ 0.01% (v/v). The reaction progress is followed by recording the loss of absorbance at 610 nm over a 10-min period (during which the velocity remained linear). Velocities are reported as the change in absorbance at 610 nm per minute, and each reported value is the average of three replicates. In experiments where the DHO or ubiquinone concentration is varied, the other substrate is held constant at 200 μM. To determine the inhibitor potency of leflunomide and A771726, the effects of varying concentrations of the two compounds on the initial velocity of the DHODase reaction is measured over a concentration range of 0.01?1.0 μM. In these experiments the DHO and ubiquinone concentrations are held constant at 200 and 100 μM, respectively. |
别名 | Fusidine, 夫西地酸 |
分子量 | 516.71 |
分子式 | C31H48O6 |
CAS No. | 6990-06-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 45 mg/mL (87.09 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.9353 mL | 9.6766 mL | 19.3532 mL | 48.383 mL |
5 mM | 0.3871 mL | 1.9353 mL | 3.8706 mL | 9.6766 mL | |
10 mM | 0.1935 mL | 0.9677 mL | 1.9353 mL | 4.8383 mL | |
20 mM | 0.0968 mL | 0.4838 mL | 0.9677 mL | 2.4192 mL | |
50 mM | 0.0387 mL | 0.1935 mL | 0.3871 mL | 0.9677 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Fusidic acid 6990-06-3 Microbiology/Virology Others oxidation-reduction Antibiotic CAT Antibacterial SQ16603 elongation inhibit Inhibitor Fusidate growth Bacterial Fusidine ribosome SQ 16603 SQ-16603 bacteriostatic steroids 夫西地酸 factor inhibitor