3-(3-Hydroxyphenyl)propionic Acid (m-Hydroxyphenylpropionic acid) is one of the major metabolites of ingested caffeic acid.it inhibited osteoclastogenesis and bone osteoclastic resorptive activity.

3-(3-Hydroxyphenyl)propionic Acid （3-3-PPA）和马尿酸（HA）在Corning骨质分析板上对成骨细胞的生成和破骨细胞的吸收活性表现出剂量依赖性的抑制效果。信号通路分析显示，经HA或3-3-PPA预处理后，RANKL刺激下的成骨细胞标记物增加，如活化T细胞的核因子、细胞质1及基质金属蛋白酶9的基因/蛋白表达被抑制。HA和3-3-PPA抑制成骨细胞生成的效果是通过RANKL/RANK独立的介体实现的[1]。

RAW264.7 cells or nonadherent bone marrow cells were cultured in 96‐well plates (2?×?10^4cells/well) in the presence or absence of 50?ng/ml of RANKL.?Cells were treated with HA at four different concentrations, 0.01×, 0.1×, 1×, and 10×, with 1× equivalent to concentration of 60?μg/dl of HA free form appeared in blood after 10% BB supplemental diet in rats.?Four different concentrations of 3‐3‐PPA or 3‐(4‐hydroxyphenyl) propionic acid (3–4‐PPA), 0.1×, 1×, 10×, and 100×, with 1× equivalent to concentration of 10?μg/dl of 3‐3‐PPA or 3–4‐PPA free form appeared in blood after 10% BB supplemental diet in rats.?After 4 days for RAW264.7 cell cultures and 5 days for bone marrow cell cultures, the cells were ?xed with 4% paraformaldehyde and stained for tartrate‐resistant acid phosphatase (TRAPase) activity using a TRAPase Staining Kit .?TRAP‐positive cells containing >3 nuclei in each well were counted as osteoclasts under an epifluorescent microscope[1]