IRAK4-IN-27 (Compound 22) is a potent and selective IRAK4 inhibitor, demonstrating an IC50 of 8.7 nM. It effectively suppresses cell growth and induces apoptosis in the MYD88 L265P diffuse large B-cell lymphoma (DLBCL) cell line, positioning it as a valuable agent for DLBCL research [1].

IRAK4:8.7 nM

IRAK4-IN-27（化合物22，72小时）对 MYD88 L265P DLBCL细胞系（OCI-LY10，IC 50 = 0.248 μM）和 MYD88野生型细胞系（U2932，IC 50 = 1.251 μM；GM00637，IC 50 = 1.520 μM）均显示出明显的抗增殖效果[1]。在0.5 μM和1 μM浓度下，经过24小时和48小时处理，IRAK4-IN-27能诱发OCI-LY10细胞的凋亡[1]。此外，IRAK4-IN-27（0.03-3 μM）能够抑制OCI-LY10细胞中IRAK4的磷酸化及其下游信号通路[1]。IRAK4-IN-27与Ibrutinib联用（0.1 μM和0.2 μM，48小时）更能有效抑制OCI-LY10细胞的生长，并促进细胞凋亡[1]。