Bleomycin Sulfate (Blenoxane) is a glycopeptide antibiotic, an inhibitor of DNA synthesis. Bleomycin Sulfate causes DNA strand breaks but not RNA strand breaks. Bleomycin Sulfate has antitumor activity.

UT-SCC-19A cells:4 nM

方法：人胰腺细胞 (PCCs) AsPC-1 和 MIA PaCa-2 用 Bleomycin Sulfate (0.1-100 µM) 处理 24-72 h，使用 MTT 方法检测细胞生长抑制情况。
结果：AsPC-1 细胞对 Bleomycin Sulfate 无反应。Bleomycin Sulfate 显著抑制了 MIA-PaCa-2 的细胞生长，在处理 24/48/72 h 时的 IC50 分别为 5.9/6.4/2.6 μM。[1]
方法：间充质干细胞 MSCs 用 Bleomycin Sulfate (1800 ng/mL) 处理 24-96 h，使用 Flow Cytometry 方法检测 sub-G1 和 caspase-3。
结果：Bleomycin Sulfate 诱导 sub-G1 和 caspase-3 的激活上调，表明 Bleomycin Sulfate 诱导细胞凋亡。[2]
方法：正常大鼠肺泡巨噬细胞用 Bleomycin Sulfate (0.01-1 μg/mL) 孵育 18 h，使用 MDGF Assay 测定其水平。
结果：Bleomycin Sulfate 刺激巨噬细胞产生成纤维细胞生长因子 (MDGF)。[3]

方法：为检测体内抗肿瘤活性，将 Bleomycin Sulfate (20 mg/kg) 腹腔注射给携带小鼠结直肠癌瘤肿瘤 CT26 shControl 或 shCRT 的 BALB/c 小鼠，每两天一次，给药五次。
结果：Bleomycin Sulfate 延迟了对照 shRNA 转染的 CT26 细胞的生长，但对 CRT shRNA 转染 CT26 细胞没有影响。[4]
方法：为研究博来霉素诱导的小鼠肺纤维化的时间过程，将 Bleomycin Sulfate (0.06 mg/只 in 0.9% saline) 单次气管内滴注 (IT) 给 C57Bl/6J 小鼠。
结果：Bleomycin Sulfate 诱导小鼠肺纤维化。该模型中评估肺纤维化的最合适的时间点是在 IT 滴注 Bleomycin Sulfate 后 14 天，这是基于观察到的动物在 14 天时出现广泛的纤维化，但纤维化反应的变异性较小，死亡率低于21天。[5]

ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference)