Theaflavin is a polyphenolic flavonoid that has been found in black tea and has diverse biological activities, including antioxidant, anticancer, anti-inflammatory, and antiviral properties

Theaflavin 抑制OVCAR-3 和 A2780/CP70 人类卵巢癌细胞系的生长，但对卵巢上皮细胞系IOSE 364 不产生抑制作用（IC50 分别为11.9、38.5 和 >40 μM）[1]。

在预处理的ICH大鼠中，Theaflavin显著缓解了行为缺陷，保护了血脑屏障（BBB）的完整性，减少了脑水肿的形成和活性氧（ROS）以及炎症细胞因子（包括白细胞介素-1β[IL-1β]、IL-18、肿瘤坏死因子-α、干扰素-γ、转化生长因子β和(C-X-C基序)配体1[CXCL1]）的水平。Nissl染色和TUNEL实验显示，Theaflavin通过抑制核转录因子kappa-β-p65（NF-κβ-p65）、caspase-1和IL-1β[2]的激活，保护神经细胞免受损失和凋亡。

Cell proliferation assay, Hoechst 33342 staining assay, Caspase-Glo Assay, western blot, human umbilical vein endothelial cell tube formation assay and vascular endothelial growth factor (VEGF) enzyme-linked immunosorbent assay were performed[1].

ICH rat models were induced with type VII collagenase and pretreated with TF by gavage in different doses (25 mg/kg-100 mg/kg).?Twenty-four hours after ICH attack, evaluated the rats' behavioral performance, the blood-brain barrier (BBB) integrity, and the formation of cerebral edema.?The levels of reactive oxygen species (ROS) and inflammatory cytokines were examined by 2',7'-dichlorofluorescin diacetate and enzyme-linked immunosorbent assay.?Nissl staining and transferase dUTP nick end labeling (TUNEL) were aimed to detect the neuron loss and apoptosis, the mechanism of which was explored by Western blot[2].