Sobetirome (IACS-010759) is a selective agonist of thyroid hormone receptor β (TRβ) and binds selectively to TRβ-1 (EC50: 0.16 μM).

TRα 1:0.58 μM (EC50), TRβ 1:0.16 μM (EC50)

GC-1特意设计用于选择性地结合于TRβ（EC50：TRα-1为0.58 μM，TRβ-1为0.16 μM）[1]。作为甲状腺激动剂，GC-1通过Wnt/β-catenin信号通路诱导肝细胞增殖，可能在急性及慢性肝功能不全中促进再生[2]。

注射四周后，小鼠被喂养含5 mg/kgSobetirome或基础饮食，持续10天或21天。Sobetirome的治疗在10天或21天内显著降低了肿瘤负担[2]。Sobetirome治疗使得标准饮食下的小鼠血清胆固醇水平降低了25%，血清甘油三酯降低了75%，同时减轻了饮食引起的高胆固醇血症[3]。Sobetirome（50或100 mug/100 g体重）显著促进了大鼠肝细胞在无组织损伤的情况下的增殖，并导致了大量的胰腺细胞增殖[4]。

Briefly, 20 μg of a pT3-EF5α-hMet-V5 and pT3-EF5α-S45Y-β-catenin-Myc combination along with the transposase in a ratio of 25:1 were diluted in 2 mL of normal saline (0.9% NaCl), filtered through a 0.22-μm filter, and injected into the lateral tail vein of 23 FVB mice that were around 6 weeks old, in 5 to 7 seconds. These mice are referred henceforth as hMet-mutant-β-catenin mice. Four weeks after injection, hMet-mutant-β-catenin mice were randomized into two groups. One group was kept on a basal diet (n = 12), and another group was switched to a GC-1–supplemented diet (5 mg/kg of diet) (n = 11). Animals on control diet were sacrificed at either 21 days (n = 8) or 10 days (n = 4) after initiation of the diet. Similarly, animals on the GC-1 diet were sacrificed at either 21 days (n = 7) or 10 days (n = 4) after initiation of the diet. The animals were given access to food and water ad libitum with a 12-hour light/dark daily cycle. One intraperitoneal injection of bromodeoxyuridine (BrdU) was performed on day 9 during 10 days of GC-1 or basal diet treatment, and livers were harvested 24 hours later [2].