Paradol ([6]-Gingerone) is a pungent phenolic substance found in ginger and other Zingiberaceae plants, and exhibits a variety of biological activities including anti-cancer, anti-inflammatory, and anti-oxidative activities,neuroprotective Effects.

EAE症状型小鼠自免疫后第29天起，每日一次口服Paradol或6-帕拉多（5 mg/kg），持续14天（至第42天）。自给药第四天起（第32至42天），除了6-帕拉多处理组的EAE小鼠在第36和37天外，Paradol和6-帕拉多显著改善了EAE相关症状，与对照组（车用处理组）相比（图1A，补充表1）。从第30天到第43天的累积临床评分也清楚地显示了Paradol和6-帕拉多的有效性。6-帕拉多或Paradol的给药显著降低了累积临床评分（Paradol，25.25%；6-帕拉多，25.75%），与对照组相比。Paradol及其代谢物6-帕拉多，对EAE具有神经保护作用。此外，这些分子对EAE具有治疗效果[1]。

MOG35-55 was emulsified in an equal amount of CFA.?Mice were anesthetized with isoflurane and 200 μg of emulsion MOG35-55 in CFA was injected subcutaneously at the start day of immunization (day 1). In addition, 400 ng of Bordetella pertussis toxin (PTX) per mouse was injected intraperitoneally on the start day of MOG immunization and 2 days later.?Mice were weighed and monitored daily for clinical symptoms of EAE as follows: 0, no clinical signs of EAE;?0.5, some lack of tone, however, some strength at the base of tail;?1.0, total loss of tail tonicity and flaccid tail;?2.0, hind limb weakness;?2.5, incomplete paralysis of one or both hind limbs;?3.0, total paralysis of one or both hind limbs;?4.0, hind and fore limbs paralysis;?5.0, death from disease.?For drug administration, symptomatic EAE mice were divided into three groups;?(1) the vehicle-treated EAE group, (2) the 6-shogaol-treated EAE group, and (3) the 6-paradol-treated EAE group.?Vehicle (10% Tween 80), 6-shogaol (5 mg/kg), or 6-paradol (5 mg/kg) was orally administered daily into symptomatic EAE mice from day 29 to day 42 (for 13 days) post immunization[1].