MS21570 as a GPR171 antagonist based on its ability to block,with an IC50 of 220 Nm,that reduces anxiety-like behavior and fear conditioning in mice.

GPR171:220 nM

GPR171在焦虑样行为或恐惧条件反射中的作用，通过系统性或BLA内部注射MS21570，以及通过BLA中GPR171的慢病毒介导的敲除来评估。系统性注射MS21570可减轻焦虑样行为，而BLA内部注射或BLA中GPR171的敲除则能减少焦虑样行为和恐惧条件反射。

Mice were habituated to the testing room 1?h prior to each experiment.?Behaviors were videotaped and analyzed using Noldus EthoVision XT or by an experimenter blinded to the treatment groups.?Mice were injected with MS21570 (5?mg/kg) 15?min prior to elevated plus maze, open field, forced swim, and contextual fear conditioning.?For microinjection studies, MS21570 was injected 10?min prior to testing and immediately following the contextual fear conditioning.?A counterbalanced within-subjects design was used when appropriate (battery of anxiety tests).