ML241 hydrochloride

产品编号 T4684Cas号 2070015-13-1

别名

ML241 hydrochloride 是一种有效且选择性的 p97 ATPase 抑制剂，IC50值为 100 nM。

产品编号 T4684Cas号 2070015-13-1

ML241 hydrochloride 是一种有效且选择性的 p97 ATPase 抑制剂，IC50值为 100 nM。

大包装 & 定制

加入购物车

实验操作小课堂

选择批次:

纯度:99.96%

联系我们获取更多批次信息

资源下载

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

生物活性

产品描述	ML241 is a potent and selective inhibitors of p97 ATPase.ML241 inhibits degradation of a p97-dependent but not a p97-independent proteasome substrate in a dual-reporter cell line. ML241 may be a novel agent for the chemotherapy of cancer, and provide a rationale for developing pathway-specific p97 inhibitors.
靶点活性	p97:100 nM
体外活性	ML241对HCT15和SW403细胞表现出细胞毒性，其GI50分别为经过24小时处理后的53μM和33μM，以及经过72小时处理后的13μM和12μM。
细胞实验	HeLa cells stably expressing ODD-luciferase are seeded onto a 96-well white solid bottom plate (5000 cells/well) and cells are grown for 16 h. Cells are treated with DMEM containing MG132 (4 μM) for 1h and washed with 100 μL PBS twice. DMEM containing 2.5% FBS, cycloheximide (50 μg/mL) and ML241 are added into the well. Four 96-well plates are prepared and one of the plates is taken out from incubator at each time point (70, 90, 120, or 150 min). Luciferin (50 μL of 1 mg/mL in PBS) is added into each well containing 50 μL of medium and incubated at room temperature with shaking at 500 rpm for 5 min. Luminescence intensity is determined with 0.1 ms integration time on the Synergy HT Microplate Reader

化学信息

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

溶解度信息

DMSO: 50 mg/mL (122.27 mM), Sonication is recommended.

溶液配制表

DMSO