Dihydrostreptomycin sulfate (Dihydrostreptomycin sesquisulfate) inhibits protein synthesis by binding to the 30S ribosomal subunit. Dihydrostreptomycin Sulfate is a semi-synthetic aminoglycoside antibiotic with bactericidal properties. This antibiotic is active against most gram-positive and gram-negative organisms and is used in the treatment of tuberculosis and tularemia.

抗生素Dihydrostreptomycin 的主要作用机制是与细菌核糖体结合并改变其功能，从而导致蛋白质翻译的减少和异常。它通过与MscL(大通道机械敏感通道)的特定位点结合并改变其构象来进入细菌细胞，因此允许K+和谷氨酸从细胞内流出，同时将Dihydrostreptomycin引入细胞内[1]。

Dihydrostreptomycin sulfate 会导致小鼠模型耳蜗损伤，而在Dihydrostreptomycin sulfate 诱发损伤后，螺旋韧带中的细胞可能会发生增殖。这一过程可能与耳蜗功能的恢复有关[2]。

Briefly, the peptide substrate N-acetyl-Asp-Glu-Val-Asp-ρ-nitroanilide (Ac-DEVD-ρNA) is added to the cell lysates in assay buffer (50 mM HEPES, pH 7.4, 100 mM NaCl, 0.1% CHAPS, 10 mM dithiothreitol, 1 mM EDTA, 10% glycerol) and incubated at 37°C. The cleavage of the substrate is monitored at 405 nm.