Bradykinin (acetate) is an active peptide that is generated by the kallikrein-kinin system. It is a inflammatory mediator and also recognized as a neuromediator and regulator of several vascular and renal functions. Enzymatically produced from kallidin in the blood, it is a powerful vasodilator that causes smooth muscle contraction, and may mediate inflammation. It has a role as a human blood serum metabolite and a vasodilator agent. It is a tautomer of a bradykinin(2+).

外源性布拉地肽显著地以剂量依赖的方式抑制了LPS诱导的mRNA和蛋白水平的TF表达。此外，NO合酶拮抗剂L-NAME和PI3K抑制剂LY294002大幅度消除了布拉地肽对组织因子表达的抑制效果。通过布拉地肽引导的PI3K/Akt信号通路激活降低了GSK-3β和MAPK的活性，并减少了核内NF-κB水平，从而抑制TF表达。在C57/BL6小鼠腹腔内注射布拉地肽也抑制了下腔静脉结扎引起的血栓形成。

To study the effect of bradykinin on TF expression, we used Lipopolysaccharides (LPS) to induce TF expression in human umbilical vein endothelial cells and monocytes.?Transcript levels were determined by RT-PCR, protein abundance by Western blotting.?In the in vivo study, bradykinin and equal saline were intraperitoneally injected into mice for three days ahead of inferior cava vein ligation that we took to induce thrombus formation, after which bradykinin and saline were injected for another two days.?Eventually, the mice were sacrificed and tissues were harvested for tests[1].