N6022(IC50 of 8 nM) is an effective, reversible, and selective S-Nitrosoglutathione reductase(GSNOR) inhibitor.

GSNOR:8 nM

N6022以浓度依赖的方式与大鼠血浆蛋白结合。在较低的化合物浓度(20 μM)下，N6022对ATP的影响大于对GSH的影响。N6022（IC50为8 nM，Ki为2.5 nM）像竞争性抑制剂一样与GSNO底物结合口袋结合。N6022与辅因子NAD+和NADH无竞争关系。

N6022以50 mg/kg的剂量处理大鼠，导致肉芽肿发生率略有增加。在血清中，N6022的溶解浓度可达5 mg/mL。

N6022 is dissolved in 5% DMSO. N6022 is tested using a rat hepatoma (H4IIE) cell line whereby cells are seeded into 96-well plates and cultured in medium containing 20% bovine serum. Following an equilibration period of 48 h, the cells are treated with N6022 (5% DMSO vehicle) at concentrations of 0, 1, 5, 10, 20, 50, 100, and 300 μM for 24 h at 37°C in 5% CO2. Camptothecin and rotenone are included as positive controls. The cell supernatant or the cells themselves are harvested for biochemical analysis.