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IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPJ-00036

IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 16-18 KDa and the accession number is P60568.

IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated

IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPJ-00036
IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 16-18 KDa and the accession number is P60568.
规格价格库存数量
20 μg¥ 2,2605日内发货
100 μg¥ 6,1305日内发货
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产品信息

生物活性
1. Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Biotinylated Human IL-2-His-Avi with an affinity constant of 8.60 nM as determined in BLI assay. (Regularly tested) 2. Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.2 ng/ml. (Regularly tested)3. Immobilized Human IL-2RA-His at 2μg/ml (100 μl/well)can bind Human IL-2-His-Avi. The ED50 of Human IL-2-His-Avi is 6.45 ng/ml. (Regularly tested)
产品描述
IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 16-18 KDa and the accession number is P60568.
种属
Human
表达系统
HEK293 Cells
标签C-6xHis-Avi
蛋白编号P60568
别名
白细胞介素,白介素,TCGF,T-Cell Growth Factor,Interleukin-2,IL-2,IL2,Aldesleukin
氨基酸序列
Ala21-Thr153
蛋白构建
Ala21-Thr153
蛋白纯度
Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
分子量16-18 KDa (reducing condition)
内毒素< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Interleukin-2(IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system,belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.

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