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APRIL/TNFSF13 Trimer Protein, Human, Recombinant (His & Avi & Flag), Biotinylated
产品编号 TMPK-00140
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL/TNFSF13 Trimer Protein, Human, Recombinant (His & Avi & Flag), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi and N-Flag tag. The predicted molecular weight is 52.1 kDa and the accession number is O75888-1.
APRIL/TNFSF13 Trimer Protein, Human, Recombinant (His & Avi & Flag), Biotinylated
产品编号 TMPK-00140
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL/TNFSF13 Trimer Protein, Human, Recombinant (His & Avi & Flag), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi and N-Flag tag. The predicted molecular weight is 52.1 kDa and the accession number is O75888-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 5,180 | 5日内发货 | |
| 500 μg | ¥ 20,700 | 5日内发货 | |
| 1 mg | ¥ 34,600 | 5日内发货 |
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生物活性
| 生物活性 | Immobilized Human BCMA, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA. |
| 产品描述 | The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL/TNFSF13 Trimer Protein, Human, Recombinant (His & Avi & Flag), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi and N-Flag tag. The predicted molecular weight is 52.1 kDa and the accession number is O75888-1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | N-His-Avi, N-Flag |
| 蛋白编号 | O75888-1 |
| 别名 | APRIL,TRDL1,TALL2,TALL-2,CD256,TNFSF13,ZTNF2,2310026N09Rik,TRDL-1,APRILFLJ57090 |
| 蛋白构建 | Lys112-Leu250 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| 分子量 | 52.1 kDa (predicted). Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. |
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