Oxazolone has been used as a haptenizing agent to induce inflammatory responses in intestinal tissue of adult zebrafish and as a model for gene expression studies in two forms of inflammatory bowel disease, such as Crohn's disease and ulcerative colitis.

Oxazolone诱导的AD样皮肤炎症模型：
在第一次攻击前7天，首先在腹部用3%Oxazolone（100μl）对小鼠进行表皮致敏，并每隔一天重复攻击，分别在右耳和左耳上局部施用0.5%Oxazolone（10μl）。[3]
Oxazolone诱导的溃疡性结肠炎模型：
1.首先用甲氧芬轻微麻醉小鼠，然后通过3.5 F的导管和1ml注射器每直肠给药6 mg的耦合剂Oxazolone；
2.导管插入至肛门边缘近端4cm处，注射1% Oxazolone, 50%乙醇溶液，总容积150μL。
3.为了保证Oxazolone在整个结肠和盲肠内的分布，注射后小鼠保持垂直姿势30 s。[4]