IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1.

Human

HEK293 Cells

白细胞介素,白介素,P35,NKSF1,NFSK,Interleukin-35,interleukin 12A,IL-35,IL-12A,CLMF

A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a C-terminal Fc region of human IgG1 tag followed by a His tag, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Arg 23 & Arg 21

> 90 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

IL12A is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.