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B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 52.4 kDa and the accession number is Q9Y275-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,250 | 现货 | |
500 μg | ¥ 13,000 | 5日内发货 | |
1 mg | ¥ 21,700 | 5日内发货 |
生物活性 | 1. Immobilized Human BAFF Trimer, His Tag at 2 μg/mL (100 μL/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9 ng/mL determined by ELISA._x000D_
2. Immobilized Human BAFF Trimer, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5 ng/mL determined by ELISA._x000D_
3. Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). |
产品描述 | B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 52.4 kDa and the accession number is Q9Y275-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-His-Flag |
蛋白编号 | Q9Y275-1 |
别名 | ZTNF4,TNFSF20,TNFSF13B,THANK,TALL1δ4 BAFF,TALL-1δ BAFF,TALL1Delta4 BAFF,TALL-1delta BAFF,TALL-1,TALL1,DTL,CD257,BLyS,BAFF |
蛋白构建 | Thr141-Leu285 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 52.4 kDa (predicted). Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
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