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Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Placental type) /ALPP Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 53.69 kDa and the accession number is XP_045223825.1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | |
500 μg | ¥ 12,400 | 5日内发货 | |
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase (Placental type) , His Tag with an affinity constant of 0.11 μM as determined in SPR assay. |
产品描述 | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Placental type) /ALPP Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 53.69 kDa and the accession number is XP_045223825.1. |
种属 | Cynomolgus |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | XP_045223825.1 |
别名 | Alkaline phosphatase,PLAP,PLAP-1,ALPP |
蛋白构建 | Ile21-Thr503 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 53.69 kDa (predicted). Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
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