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Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag)

产品编号 TMPK-00796

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 54.5 kDa and the accession number is P09923.

Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag)

Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag)

产品编号 TMPK-00796
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 54.5 kDa and the accession number is P09923.
规格价格库存数量
100 μg¥ 3,7005日内发货
500 μg¥ 14,8005日内发货
1 mg¥ 24,7005日内发货
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生物活性

生物活性
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 μM as determined in SPR assay.
产品描述
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 54.5 kDa and the accession number is P09923.
种属
Human
表达系统
HEK293 Cells
标签N-His-Flag
蛋白编号P09923
别名
FLJ40094,ALPI,APTNAP,EC 3.1.3.1,AP-TNAP,IAP,TNAP,HOPS,FLJ93059,MGC161443,TNSALP,Akp2
蛋白构建
Val20-Asp503
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量54.5 kDa (predicted). Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

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