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Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPK-00801

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 55.48 kDa and the accession number is P10696.

Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His & Avi), Biotinylated

Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPK-00801
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 55.48 kDa and the accession number is P10696.
规格价格库存数量
100 μg¥ 5,1805日内发货
500 μg¥ 20,7005日内发货
1 mg¥ 34,6005日内发货
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生物活性

生物活性
Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 23.4ng/ml determined by ELISA.
产品描述
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 55.48 kDa and the accession number is P10696.
种属
Human
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号P10696
别名
ALPPL2,PLAP-like,GCAP,ALPG,Alkaline phosphatase, placental-like,ALPPL,Alkaline Phosphatase,ALP-1
蛋白构建
Ile20-Asp503
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
分子量55.48 kDa (predicted). Due to glycosylation, the protein migrates to 56-70 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

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