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SARS-COV-2 Spike S Trimer Protein (His & Avi), Biotinylated
产品编号 TMPK-00441
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S Trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 136.6 kDa and the accession number is A0A6G7K2L4.
SARS-COV-2 Spike S Trimer Protein (His & Avi), Biotinylated
产品编号 TMPK-00441
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S Trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 136.6 kDa and the accession number is A0A6G7K2L4.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 5,930 | 5日内发货 | |
| 500 μg | ¥ 23,700 | 5日内发货 | |
| 1 mg | ¥ 39,500 | 5日内发货 |
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产品信息
| 生物活性 | Immobilized Biotinylated SARS-COV-2 Spike S (Trimer) , His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 91.1ng/ml determined by ELISA. |
| 产品描述 | The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S Trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 136.6 kDa and the accession number is A0A6G7K2L4. |
| 种属 | SARS-CoV-2 |
| 表达系统 | HEK293 Cells |
| 标签 | C-His-Avi |
| 蛋白编号 | A0A6G7K2L4 |
| 别名 | Spike glycoprotein,S protein,S glycoprotein,COVID-19 |
| 蛋白构建 | Val16-Glu1188 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
| 分子量 | 136.6 kDa (predicted). Due to glycosylation, the protein migrates to 140-170 kDa based on Tris-Bis PAGE result. |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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