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The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 (Omicron B.1.1.529) Spike S Trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 136.70 kDa and the accession number is A0A6G7K2L4.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 5,930 | 5日内发货 | |
500 μg | ¥ 23,700 | 5日内发货 | |
1 mg | ¥ 39,500 | 5日内发货 |
生物活性 | Immobilized Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. |
产品描述 | The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 (Omicron B.1.1.529) Spike S Trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 136.70 kDa and the accession number is A0A6G7K2L4. |
种属 | SARS-CoV-2 |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | A0A6G7K2L4 |
别名 | S glycoprotein,COVID-19,S protein,Spike glycoprotein |
蛋白构建 | Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 136.70 kDa (predicted). Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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