NGF Protein, Human, Recombinant (HEK293)

产品编号 TMPY-06985

NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.8 kDa. and the accession number is P01138.

NGF Protein, Human, Recombinant (HEK293)

产品编号 TMPY-06985

NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.8 kDa. and the accession number is P01138.

规格	价格	库存	数量
100 μg	¥ 1,350	现货

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产品信息

生物活性	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-20 ng/mL.
产品描述	NGF Protein, Human, Recombinant (HEK293) is expressed in HEK293 mammalian cells. The predicted molecular weight is 24.8 kDa. and the accession number is P01138.
种属	Human
表达系统	HEK293 Cells
标签	Tag Free
蛋白编号	P01138
别名	神经生长因子,β-NGF,NGFB,nerve growth factor (β polypeptide),nerve growth factor (beta polypeptide),HSAN5,Beta-NGF
蛋白构建	A DNA sequence encoding the human NGF (P01138, with mutation Arg 121 Gly) (Met1-Ala241) was expressed.
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	The recombinant human NGF consists of 223 amino acids and predicts a molecular mass of 24.8 kDa.
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. . Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.