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LIF Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The predicted molecular weight is 19.9 kDa and the accession number is P09056.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
5 μg | 待询 | 期货 | |
100 μg | 待询 | 期货 | |
500 μg | 待询 | 期货 |
生物活性 | Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures. |
产品描述 | LIF Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The predicted molecular weight is 19.9 kDa and the accession number is P09056. |
种属 | Mouse |
表达系统 | E. coli |
标签 | Tag Free |
蛋白编号 | P09056 |
别名 | LIF,Differentiation-stimulating factor,D factor |
氨基酸序列 | SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF |
蛋白构建 | 24-203 aa |
蛋白纯度 | > 98% as determined by SDS-PAGE and HPLC. |
分子量 | 19.9 kDa (Predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02% Tween 20. |
复溶方法 | Reconstitute the lyophilized protein in sterile deionized water containing 0.1% BSA. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |