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LIF Protein, Mouse, Recombinant (E. coli)

产品编号 TMPX-00003

LIF Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The predicted molecular weight is 19.9 kDa and the accession number is P09056.

LIF Protein, Mouse, Recombinant (E. coli)

LIF Protein, Mouse, Recombinant (E. coli)

产品编号 TMPX-00003
LIF Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The predicted molecular weight is 19.9 kDa and the accession number is P09056.
规格价格库存数量
5 μg 待询 期货
100 μg 待询 期货
500 μg 待询 期货
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产品信息

生物活性
Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 × 108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.
产品描述
LIF Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The predicted molecular weight is 19.9 kDa and the accession number is P09056.
种属
Mouse
表达系统
E. coli
标签Tag Free
蛋白编号P09056
别名
LIF,Differentiation-stimulating factor,D factor
氨基酸序列
SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
蛋白构建
24-203 aa
蛋白纯度
> 98% as determined by SDS-PAGE and HPLC.
分子量19.9 kDa (Predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02% Tween 20.
复溶方法
Reconstitute the lyophilized protein in sterile deionized water containing 0.1% BSA. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

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