IL-10 Protein, Human, Recombinant (His & Avi)

产品编号 TMPK-00056

Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. IL-10 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 21.3 kDa and the accession number is P22301.

IL-10 Protein, Human, Recombinant (His & Avi)

产品编号 TMPK-00056

规格	价格	库存
100 μg	¥ 4,440	5日内发货
500 μg	¥ 17,800	5日内发货
1 mg	¥ 29,600	5日内发货

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产品信息

生物活性	1. Immobilized Human IL-10, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA. 2. Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay.
产品描述	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. IL-10 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 21.3 kDa and the accession number is P22301.
种属	Human
表达系统	HEK293 Cells
标签	N-His-Avi
蛋白编号	P22301
别名	白细胞介素,白介素,TGIF,MGC126451,MGC126450,Interleukin-10,IL10A,IL-10,GVHDS,CSIF,B-TCGF
蛋白构建	Ser19-Asn178
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	21.3 kDa (predicted). Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.