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FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
10 μg | ¥ 560 | 现货 | |
20 μg | ¥ 780 | 5日内发货 | |
50 μg | ¥ 1,240 | 现货 | |
100 μg | ¥ 1,750 | 5日内发货 |
生物活性 | 1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 , VEGFA , EGF . Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested)
2.Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.01-0.1 ng/mL. |
产品描述 | FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4. |
种属 | Human |
表达系统 | E. coli |
标签 | Tag Free |
蛋白编号 | P09038-4 |
别名 | HBGF-2,fibroblast growth factor 2 (basic),FGFB,FGF-2,BFGF |
蛋白构建 | A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro143-Ser288) was expressed with an additional Met at the N-terminus. Predicted N terminal: Met |
蛋白纯度 | ≥ 95 % as determined by SDS-PAGE |
分子量 | 16.5 kDa (predicted) |
内毒素 | < 10 EU/mg of the protein. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstituted with sterile deionized water to 0.15 mg/mL. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.bFGF/FGF2 Protein & Antibody Products |
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