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CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment. CD161 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 19.6 kDa and the accession number is Q12918-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,400 | 5日内发货 | |
500 μg | ¥ 13,600 | 5日内发货 | |
1 mg | ¥ 22,700 | 5日内发货 |
生物活性 | 1. Immobilized Human CD161, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA (QC Test).
2. Anti-CD161 Antibody, hFc tag captured on CM5 Chip via Anti-hFc IgG can bind Human CD161, His Tag with an affinity constant of 78.8 nM as determined in SPR assay. |
产品描述 | CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment. CD161 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 19.6 kDa and the accession number is Q12918-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q12918-1 |
别名 | NKRP1ANKR,NKR-P1A,NKRP1A,NKR-P1,NKRP1,NKR,Ly59,KLRB1,HNKR-P1a,CLEC5B,CD161 |
蛋白构建 | Gln67-Ser225 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 19.6 kDa (predicted). Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment. |
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