B7-H3 Protein, Human, Recombinant (His), AF488-Labeled

产品编号 TMPY-07059

B7-H3 Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.

B7-H3 Protein, Human, Recombinant (His), AF488-Labeled

产品编号 TMPY-07059

B7-H3 Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.

规格	价格	库存	数量
25 μg	¥ 3,430	期货
100 μg	¥ 10,900	期货

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生物活性	1.5e5 of 293-CAR cells expressing the anti-B7-H3 scFv can be directly stained with 100 μL (2 μL stock solution diluted to 100 μL FCM buffer) of B7-H3 Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-07059) and the percentage of CAR-expressing cells can be detected by flow cytometry. Non-transduced 293 cells was used as a control (QC tested). 2.5e5 of PBMCs can be stained with anti-CD3 antibody and B7-H3 Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-07059) and detected by flow cytometry (QC tested). 3.Flow cytometric analysis of anti-B7-H3 CAR expression. HEK293 cells were lentivirally transduced with anti-B7-H3 CAR. Flow cytometric analysis was performed with a negative control protein and B7-H3 Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-07059), respectively. Non-transduced 293 cells were used as a control (left). 4.Binding activity of PE-conjugated B7-H3 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and B7-H3 Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-07059) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
产品描述	B7-H3 Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q5ZPR3-1
别名	UNQ309/PRO352,PSEC0249,B7RP-2,B7-H3,B7H3,4Ig-B7-H3
蛋白构建	A DNA sequence encoding the human B7-H3 (Q5ZPR3-1) (Met1-Thr461) was expressed with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
蛋白纯度	≥ 90% as determined by SDS-PAGE.
分子量	50 kDa (predicted)
内毒素	< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液	This product is Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法	Please refer to the lot-specific COA.
存储	Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

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