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B7-2 Protein, Human, Recombinant (hFc & Avi), Biotinylated

产品编号 TMPY-07031

B7-2 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is NP_008820.4.

B7-2 Protein, Human, Recombinant (hFc & Avi), Biotinylated

B7-2 Protein, Human, Recombinant (hFc & Avi), Biotinylated

产品编号 TMPY-07031
B7-2 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is NP_008820.4.
规格价格库存数量
20 μg
¥ 3,820
期货
100 μg
¥ 8,830
期货
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产品信息

生物活性
Immobilized CTLA-4 Protein, Human, Recombinant (His) (Cat#TMPY-04348) at 2 μg/mL (100 μl/well) can bind B7-2 Protein, Human, Recombinant (hFc & Avi), Biotinylated (Cat#TMPY-07031), the EC50 is 3.5-11.0 ng/mL.
产品描述
B7-2 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is NP_008820.4.
种属
Human
表达系统
HEK293 Cells
标签C-hFc-Avi
蛋白编号NP_008820.4
别名
LAB72,CD28LG2,B7-2,B70,B7.2
蛋白构建
A DNA sequence encoding the Human CD86 (NP_008820.4) (Met1-His239) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度
≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
分子量53.7 kDa (predicted); 92 kDa (reducing contition, due to glycosylation)
内毒素< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

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