XJB-5-131 is a synthetic, bi-functional antioxidant specifically designed to target mitochondria for the scavenging of reactive oxygen species (ROS) and electrons, thereby acting as a radical scavenger. Additionally, it serves as an effective protector and mitigator against ionizing irradiation for cord blood mononuclear cells (CB MNCs).

XJB-5-131 also ameliorates hemorrhagic shock (HS)-induced activation of the pro-apoptotic enzymes, caspases 3 and 7, in ileal mucosa[1]. XJB-5-131 reduces apoptosis and enhances cell survival in mouse embryonic cells?in vitro[2]. XJB-5-131 is a radiation protector for colony-forming unit-granulocyte macrophage (CFU-GM). XJB-5-131 is an effective mitigator when added after irradiation[3]. Cell Viability Assay[3]Cell Line: Low density mononuclear cells (MNC) Concentration: 10 μM Incubation Time: Added to cells one hour before irradiation or immediately after irradiation Result: Was a protector when given before irradiation as shown by an increase in the D0?to 1.93±0.13 for CFU-GM with XJB-5-131.

XJB-5-131 ameliorates peroxidation of the mitochondrial phospholipid, cardiolipin, in ileal mucosal samples from rats subjected to hemorrhagic shock (HS)[1]. Intravenous treatment with XJB-5-131 (2 μmol/kg) significantly prolongs the survival of rats subjected to profound blood loss (33.5 mL/kg) despite administration of only a minimal volume of crystalloid solution (2.8 mL/kg) and the absence of blood transfusion[1]. XJB-5-131 reduces oxidative damage to mitochondrial DNA, maintains mitochondrial DNA copy number, suppresses motor decline and weight loss, enhances neuronal survival, and improves mitochondrial function. XJB-5-131 significantly suppresses the disease phenotypes and improves mitochondrial function in a mouse model of Huntington’s disease (HD)[2]. XJB-5-131 (1 mg/kg; intraperitoneally injected; three times a week up to 57 weeks) suppresses decline of weight loss and motor function in a mouse model of HD[2]. Animal Model: Male specific pathogen-free Sprague-Dawley rats, weighing 150 to 250 g[1]Dosage: 2 μmol/kg Administration: Administered intravenously using a syringe pump Result: The rats treated with XJB-5-131 survived significantly longer (P?< 0.01). Three of six survived for longer than 3 hours after completion of the hemorrhage protocol and one rat survived for the whole 6 hours postbleeding observation period. Animal Model: HD150KI mice[2]Dosage: 1 mg/kg Administration: Intraperitoneally injected; three times a week up to 57 weeks Result: Chronic treatment suppressed weight loss. Increased the average body mass by 22%.