WKYMVM is a N-formyl peptide receptor (FPR1) agonist.

中性粒细胞通过FPRL1被WKYMVM激活，并且FPRL2是一个在髓系细胞中传导信号的趋化性受体[1]。

在采用了28位急性白血病(AL)患者和31位健康对照者的血液样本进行研究中，发现在没有外源性WKYMVM的情况下，AL患者与对照者之间的WKYMVM受体和杀菌活性无差异。添加WKYMVM显著以剂量依赖的方式增强了这些细胞的杀菌活性。在诱导化疗期间，在1nM WKYMVM的存在和缺失状态下，杀菌活性显著增加，完全缓解时的杀菌活性高于诊断时或第15天时的杀菌活性。在巩固化疗期间，WKYMVM对杀菌活性无影响。患者显示TNF alpha, IL-1b, IL-6和IL-8的浓度显著增加，但IL-2, IL-4和IL-12的浓度显著减少。TNF alpha, IL-1b和IL-6与诊断时患者中性粒细胞的杀菌活性呈现显著的负相关性，而IL-4与杀菌活性显示出显著的正相关性，这些发现表明WKYMVM在AL患者中增强了杀菌活性。

Ligand-mediated internalization of FPRL1 and FPRL2 was followed by an indirect immunofluorescence microscopy staining technique.?Cells were seeded on polyornithine-coated coverslips 48 h prior to the experiments.?To stop protein synthesis, cells were incubated in RPMI 1640 medium containing 1% BSA and 100 μg/ml cycloheximide for 3 h and maintained in the same medium throughout the experiment.?To study receptor internalization, RINm5F-FPRL1 cells and RINm5F-FPRL2 cells were treated with either WKYMVM or WKYMVm at 37 °C for various time periods ranging from 0 to 30 min. Cells were finally fixed and permeabilized with acetone at ?20 °C for 30 s[1].