Type II topoisomerase inhibitor 1 is able to be used in the antibacterial area. Type II topoisomerase inhibitor 1 is a potent and selective inhibitor of E. coli DNA gyrase with a IC50 value of 1.7 nM as well as forms hydrogen bonds with Asp73 residue. Type II topoisomerase inhibitor 1 inhibits topoisomerase IV activity with an IC50 value of 0.98 μM [1] [2].

Type II topoisomerase inhibitor 1 (13 e, 18 h) shows antibacterial activities against E. coli and S. aureus (MIC: 64 and 16 μg/mL, respectively) [1].. Type II topoisomerase inhibitor 1 (50 μM, 48 h) shows an 8.4% inhibition rate for HepG2 cells [1].. Cell Viability Assay [1] Cell Line: HepG2 cells Concentration: 10, 50 μM Incubation Time: 10, 50 μM Result: Showed cytoxicity with inhibition rates of 8.4% at 50 μM, 1.5% at 10 μM.