T-2307, a broad-spectrum arylamidine compound, demonstrates potent antifungal properties both in vitro and in vivo. Its activity extends to clinically significant pathogens such as Candida species (MIC range: 0.00025 to 0.0078 μg/ml), Cryptococcus neoformans (MIC range: 0.0039 to 0.0625 μg/ml), and Aspergillus species (MIC range: 0.0156 to 4 μg/mL).

T-2307 exhibits potent activity against fluconazole-resistant and fluconazole-susceptible-dose-dependent Candida albicans strains as well as against azole-susceptible strains[1]. T-2307 shows efficacy in a murine model of candida glabrata infection despite?in vitro?trailing growth phenomena[2]. Cell Viability Assay[2]Cell Line: C. glabrata ATCC 90030 Concentration: 0.000125, 0.00025, 0.0005, 0.001, 0.002, 0.0039, 0.0078, 0.0156, 0.0313, 0.0625, 0.125 μg/mL Incubation Time: 24 and 48 hours Result: C. glabrata exhibited significant trailing growth at concentrations between 0.0039 and 0.125 μg/mL at 48 h. The trailing growth of C. glabrata at 24 h of incubation was similar to that at 48 h.

In mouse models of disseminated candidiasis, cryptococcosis, and aspergillosis, the ED 50 of T-2307 were 0.00755, 0.117, and 0.391 mg/kg, respectively[1]. Animal Model: 4-week-old specific-pathogen-free ICR strain male mice bearing systemic infections with Candida albicans , Cryptococcus neoformans , and Aspergillus fumigatus[1]. Dosage: 0.001, 0.1, 1 mg/kg Administration: Subcutaneously administered; once a day for 7 days, beginning at 2 h after the infection. Result: In the systemic infection caused by Candida albicans , all the control mice died by day 6. Mortality was significantly delayed in mice that were administered T-2307 at a dose of 0.01 mg/kg?compared with that in the control mice. The calculated ED 50 s of T-2307were 0.00755 mg/kg. In the systemic infection caused by Cryptococcus neoformans , all the control mice died by day 9. Mortality was significantly delayed in mice administered T-2307 at a dose of 0.1 mg/kg?compared with that in the control mice. The calculated ED 50 s of T-2307 were 0.117 mg/kg. In the systemic infection caused by Aspergillus fumigatus , all the control mice died by day 6. Mortality was significantly delayed in mice that were administered T-2307 at a dose of 1 mg/kg compared with that in the control mice. The calculated ED 50 s of T-2307 were 0.391 mg/kg.