Soyasaponin Ac can decrease the drug resistance via EMT pathway and weaken the migration ability of ovarian cancer cells.

Cell viability assay was used to analyze the drug resistance of A2780/PTX cells treated with four different common soyasaponins respectively,in order to screen out the most effective soyasaponin. Then,the most effective soyasaponin was used to detect the expression of epithelial-mesenchymal transition (EMT)-related marker proteins, including N-cadherin and E-cadherin,with Western blot and confocal microscopy. Finally,transwell assay and wound healing assay were applied to observe effect of soyasaponin on regulating cancer cell migration. Compared with other soyasaponins,Soyasaponin Ac most effectively reversed the drug resistance of A2780/PTX cells. The expression of N-cadherin decreased while that of Ecadherin increased in A2780/PTX cells when treated with Soyasaponin Ac for 48 h. The results of transwell and wound healing assay suggested that Soyasaponin Ac also reduced the migration of A2780/PTX cells.