MCH (human, mouse, rat) TFA is a potent peptide agonist for MCH receptors, demonstrating strong affinity with binding IC 50 values of 0.3 nM for MCH1R and 1.5 nM for MCH2R. It is notably effective against MCH-2R in a CHO cell line, as evidenced by the mobilization of intracellular calcium measured with FLIPR, revealing functional activation EC 50 values of 3.9 nM for MCH-1R and 0.1 nM for MCH-2R [1].