D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent.

D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay[1]. D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM[1]. D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which

are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment[1]. D-3 has little influence on various non-iPSCs, including hepatocytes and neurons[1]. Cell Viability Assay[1]Cell Line: Six human iPSCs lines and one human ESC line (khES1). Concentration: 400 μM. Incubation Time: 1-2 h. Result: Sufficient to induce a viability loss (>99% in all iPSC lines and >95% in khES1).