CK2 inhibitor 3 is a highly potent inhibitor of protein kinase CK2, exhibiting an IC50 value of 280 nM. It effectively inhibits the intracellular activity of CK2 and displays a notable impact on the viability of tumor cells. Moreover, CK2 inhibitor 3 demonstrates exceptional selectivity among a diverse panel of 320 kinases [1].

CK2 inhibitor 3 (compound 4) (0-50 μM; 24 hours) significantly reduces cell viability in a dose-dependent manner, and DC 50 value is 12.80 μM [1]. CK2 inhibitor 3 (5 μM and 20 μM; 16 hours) decreases the CK2-dependent phospho-site Akt Ser129 level [1]. Cell Viability Assay Cell Line: Jurkat cells [1] Concentration: 0 μM, 10 μM, 20 μM, 30 μM, 40 μM and 50 μM Incubation Time: 24 hours Result: Significantly reduced cell viability in a dose-dependent manner, and DC 50 value was 12.80 μM. Western Blot Analysis Cell Line: Jurkat cells [1] Concentration: 5 μM and 20 μM Incubation Time: 16 hours Result: Decreased the CK2-dependent phospho-site Akt Ser129 level.